Identification of potential seed storage protein responsible for bruchid resistance in common bean landraces from Tanzania and Malawi

Bean bruchids are among the most devastating insect pests of common bean that can inflict huge losses in storage. To identify potential resistance to these pests, screening was performed at Sokoine University Agriculture. Two resistant landraces were identified, viz Kalubungula and KK25. Recombinant inbred (RI) KSy, KSw ML populations created from crosses between Soya × Kalubungula, Soworo Nagaga KK25, respectively. Seed storage proteins characterized sequenced RI population progenies determine if phenotypic associated with α-amylase inhibitor – phytohemagglutinin arcelin (APA) proteins. We found no association seed observed its recombinant lines an APA protein. KK25 had Arcelin-5, Leucoagglutinin, Erythroagglutinin a hypothetical protein conditions antibiosis effects as mechanism. The may contribute enhanced resistance.   Key words: α-amylase, phytohemagglutinin, arcelin, Acanthoscelides obtectus, Zabrotes subfasciatus, beans.